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Fig. 2 | BMC Cancer

Fig. 2

From: The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells

Fig. 2

Long-term exposure to 2DG enhances cell cycle arrest. PC3 cells were treated with 2DG for a short time (6 h) or long time (48 h). CQ was used as an autophagy inhibitor at a final concentration of 20 μM for 2 h. Rapamycin was used as an autophagy inducer at 100 nM for 6 h. a After 2DG treatment, each cell was collected for FACS analysis. Cells were stained with iodine for 5 min and analyzed for the amount of DNA content. Each DNA content rate is expressed as G1, S, and G2 phase. Data are represented as the means ± SD. *P < 0.05, **P < 0.01, **P < 0.001. (b) Expression levels of cell cycle related genes were observed using western blotting analysis

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