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Fig. 4 | BMC Cancer

Fig. 4

From: DKK1 is a potential novel mediator of cisplatin-refractoriness in non-small cell lung cancer cell lines

Fig. 4

In silico mapping of the DKK1 interactome using Ingenuity Pathway Analysis (IPA). a IPA-generated network built around DKK1 showing the direct upstream transcriptional regulators. Those that showed concurrent up-regulation along with DKK1 in replicate 1 are shown in grey. b IPA-generated network centered on DKK1 showing downstream signaling proteins that are indirectly regulated by DKK1. Those that were regulated also in replicate 1 are shown in grey. For a and b, activation (), inhibition () or reports on both (), indicates the regulation retrieved from Ingenuity. c Left panel: Q-RT-PCR showing the mRNA expression level of DKK1 in a panel of NSCLC cell lines. 18S rRNA was used as a loading control. Right panel: Correlation between baseline DKK1 mRNA expression and the cisplatin sensitivity of NSCLC cell lines after prolonged exposure (measured as area under the curve (AUC) in MTT assay after 72 h continuous treatment, based on three experiments each performed in triplicate). mRNA expression levels of RRM1 d and ERCC1 e together with correlation to cisplatin sensitivity as described in c

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