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Fig. 6 | BMC Cancer

Fig. 6

From: Chemotherapy induces Notch1-dependent MRP1 up-regulation, inhibition of which sensitizes breast cancer cells to chemotherapy

Fig. 6

Inhibition of Notch activation reduces doxorubicin-induced MRP1 function and, in T47D cells, enhances doxorubicin-dependent apoptosis. a Intracellular calcein levels in T47D cells were quantified 5 h after calcein wash-out following 24 h treatment with control, DAPT (50 μM), doxorubicin (1 μM), doxorubicin and DAPT, or 0.5 h treatment with the ABC pump-inhibitor MK-571 (25 μM). Data are presented as mean with SEM of 2 biological replicates. b Intra-cellular doxorubicin levels in T47D cells were quantified after treatment with either doxorubicin alone (1 μM for 4 h), or in combination with DAPT (pre-treated with 50 μM for 24 h) or MK-571 (pre-treated with 25 μM for 20 min). Data are presented as mean with SEM of 2 biological replicates. c Apoptosis was measured in T47D cells after doxorubicin (10nM for 24 h) and DAPT (1 μM) treatment, alone and in combination. Data are presented as mean with SEM of 2–4 biological replicates per bar. Asterisks represent significant changes compared to vehicle control or between test conditions as indicated (two tailed t-test * p < 0.05, ** p < 0.01, *** p < 0.001, ns = not significant). d-f as described for A-C but in HB2 cells

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