Fig. 4
Doxorubicin-induced expression of MRP1 in T47D cells is abrogated by inhibition of Notch1 activation. Cells were treated for 24 h with control, 1 μM doxorubicin, 50 μM DAPT (an inhibitor of Notch function) or both in combination. Left: Expression Notch1IC of was determined using ELISAs. Means with SEM of two independent biological replicates are presented. Right: MRP1 mRNA expression was quantified using qPCR. Means with SEM of two independent biological replicates are presented. T-tests were used to determine significant changes. * p < 0.05, ** <0.01, *** < 0.001, ns = not significant