Fig. 3
S100A16 mRNA expression was positively correlated with differentiation markers in OSCC specimens and in the cell fractions enriched for more differentiated cells. a–d S100A16, IVL, KRT13, TGM1 and FLG mRNA levels were obtained from external microarray dataset (Rickmen) and their correlation was examined using Pearson analysis. e–g Cell fractions were enriched for differentiated cells either by using lack of adherence to collagen IV or by FACS sorting for low p75NTR expression and mRNA expression levels of S100A16 and IVL and KRT10 were examined by qRT-PCR. e Significantly higher mRNA levels of S100A16, IVL and KRT10 were found in LAC cell fractions (enriched for more differentiated cells) as compared to RAC/MAC (enriched for less differentiated cells). Error bars represent SEM of 3 repeated experiments. ANOVA test with Bonferroni Post-Hoc was used for statistical analysis. P-value: ***, <0.001. f and g Fractions enriched for differentiated cell (p75NTRlow) expressed significantly higher expression of S100A16, IVL and KRT10 as compared to p75NTRhigh fractions in CaLH3 (f) and H357 (g) cells. Expression levels were normalized to GAPDH mRNA expression. Error bars represent SEM of 3 repeated experiments. Student’s-t test was performed for statistical analysis