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Fig. 2 | BMC Cancer

Fig. 2

From: S100A16 promotes differentiation and contributes to a less aggressive tumor phenotype in oral squamous cell carcinoma

Fig. 2

S100A16 mRNA level was progressively down-regulated from NHOM to ODL and OSCC. a S100A16 mRNA expression was examined in frozen specimens of NHOM (n = 44) and OSCCs (n = 50) by using qRT-PCR. Mean S100A16 mRNA was found to be significantly down-regulated in OSCCs (P < 0.0001). S100A16 mRNA expression levels were normalized to GAPDH mRNA expression. Error bars represent SEM. Student’s-t test was performed for statistical analysis. b–d Down-regulation of S100A16 mRNA levels in OSCC was verified in three independent microarray datasets. Error bars represent SEM. Student’s-t test was performed for statistical analysis. e Gradual down-regulation of S100A16 mRNA during the transition from NHOM to ODL and OSCC was validated in laser dissected specimens of NHOM, ODL, paratumor (dysplastic) epithelium, tumor center and invading front by qRT-PCR. qRT-PCR was done in duplicates and S100A16 mRNA level was normalized to GAPDH and ACTB mRNA levels. Error bars represent SEM. ANOVA test with Bonferroni Post-Hoc was used for the statistical analysis. P-value: ***, <0.001; **, 0.001–0.01

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