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Fig. 5 | BMC Cancer

Fig. 5

From: Neoadjuvant administration of Semliki Forest virus expressing interleukin-12 combined with attenuated Salmonella eradicates breast cancer metastasis and achieves long-term survival in immunocompetent mice

Fig. 5

Microscopic characteristics of LVR01 infected tumors. 2×107 cfu were inoculated into 4T1 tumors. Three days later, tumors were extracted, crio-preserved and analyzed. In figures a-c LVR01 is stained in red (AntiLPS Alexa 555), DNA in blue (DAPI) and cell membranes in green (WGA 488). In d-e, Gr1+ cells are shown in blue (Gr1-Alexa 488) and cell membranes are shown in green (WGA350). a Tumor regions without bacteria (I), or invaded with low (II) or high (III) density of LVR01 show a dose-dependent tissue distortion effect. b Bacterial conglomerates (arrow) indicate possible intracellular replication. c DNA staining of infected zones reveals nuclear morphologies characteristic of myeloid cells (arrows) in the proximity of LVR01 accumulation. d Gr1+ cells are present in the proximity of bacterial infected zones d Absence of granulocytes in tumoral regions without Salmonella

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