Fig. 1

CD44 and CD24 expressions of mammosphere and MCF-7 cells: Mammosphere cells and their parental MCF-7 cells were labeled for CD44 and CD24 expression. a antibody isotype controls of mammosphere cells. b mammosphere cells stained with CD44 and CD24 antibodies conjugated with FITC and PE respectively. c an image of a mammosphere cultured for 8 days, the compacted sphere contains numerous cells. d MCF-7 cells stained with isotype controls. e MCF-7 cells stained with CD44 and CD24 antibodies conjugated with FITC and PE respectively. f and g histogram diagrams to show the CD24 (f) and CD44 (g) expression shifts (the decrease of CD24 and the increase of CD44 in mammosphere cells)