Fig. 6

Over-expressing Akt enhances the anti-tumor efficacy of PBLs in vitro and in vivo. a PBLs transduced with CAR, CAR-Akt, or CAR-myr-Akt were incubated with PC3M-luc at an E:T ratio of 2:1, 24 h later, cell viability was determined by luminescence imaging, and the luminescence intensity was normalized to that of PC3M-luc cells without treatment. Statistical analysis was performed using student’s t-test. b NOD/SCID mice were subcutaneously injected with PC3M tumor cells at 6 × 10⁶ cells/mouse, at 21 days after tumor challenge, PBLs transduced with CAR, CAR-Akt, or CAR-myr-Akt were intratumorally injected into mice at 3 × 10⁶ cells/mouse, and the mice without treatment were used as control. Tumor volume was measured every few days, and statistical analysis was performed using two-way ANOVA