Fig. 5
From: Over-expressing Akt in T cells to resist tumor immunosuppression and increase anti-tumor activity
Over-expressing Akt increases the activity of CAR expressing PBLs in the presence of PC3M. PBLs transduced with CAR, CAR-Akt, or CAR-myr-Akt were co-cultured with or without PC3M at an E:T ratio of 2:1, 3Â days later, cell proliferation was examined by CFSE assay (a), and cell apoptosis was determined by Annexin V/PI staining (b), the analysis was performed with gated CD8+ population