Fig. 5

Effect of canadine on the G-quadruplex stabilization and on the RET promoter activity. a Structures of berberine and canadine. b CD titration spectra for the RET-WT (5 μM) in the absence and in the presence of canadine (5 Equivalents) with increasing temperatures from 20 °C to 90 °C. c Melting curve of the RET-WT G-quadruplex in the absence and in the presence of canadine (5 Equivalents). d Effect of canadine on the luciferase expression level in HEK293-WT cell line at various concentrations after 24 h treatment. e Effect on the RET protein expression in TT cells after 48 h incubation at an increasing concentrations of canadine