Fig. 7

HCAb2 specifically targeted a unique population of MDA-MB-231 xenograft tumor cells. a-h Immunofluorescence analysis of xenograft tumor vasculature and HCAb2 localization. 24 h time point tumor section was incubated with anti-CD31 and Alexa Fluor® 488 anti-mouse IgG antibodies. Nuclei were stained with DAPI. (a-d) Representative images depicting a region in the tumor that shows HCAb2 localization and positive CD31 staining. (e-h) Representative images depicting a different region in the same tumor showing no HCAb2 localization but positive CD31 staining. Scale bar represents 20 μm. i-l Immunofluorescence detection of HSP90 and HCAb2 in xenograft tumor cells. 24 h time point tumor section was incubated with anti-HSP90 and Alexa Fluor® 488 anti-mouse IgG antibodies. Isolated HCAb2 localization was observed (panel j) while uniform HSP90 staining was observed (panel k) throughout the tumor section. Scale bar represents 10 μm. m-p Immunofluorescence detection of calnexin and HCAb2 in xenograft tumor cells. 24 h time point tumor section was incubated with anti-calnexin and Alexa Fluor® 488 anti-mouse IgG antibodies. HCAb2 localized to MDA-MB-231 cells that lacked calnexin staining as seen from panels n-p. Scale bar represents 10 μm