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Fig. 4 | BMC Cancer

Fig. 4

From: Patient-derived heavy chain antibody targets cell surface HSP90 on breast tumors

Fig. 4

Validation of target antigens of HCAb1 and HCAb2. a Validation of HSP90 to be the target antigen of HCAb2. Immunoprecipitation of HSP90 was performed from cytosolic and plasma membrane (PM) fractions using HCAb2. Immunoprecipitated HSP90 was detected using a commercial anti-total HSP90 antibody. b Immunoprecipitation of recombinant human HSP90β protein using HCAb1 and HCAb2. HCAb1 and HCAb2 (5 μg each) were used to immunoprecipitate recombinant HSP90β (1 μg) resuspended in either 1 % Triton X-100 + 0.1 % SDS or 1 % Triton X-100 + 1 % SDS buffers. Immunoprecipitated HSP90β protein was detected using a commercial anti-total HSP90 antibody. Equal amounts of HCAb1 and HCAb2 were pulled down as detected by anti-mouse IgG antibody

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