Fig. 2

HCAb2 bound strongly to primary breast tumor tissues in comparison to normal breast tissues. a-h Immunofluorescence analysis of primary breast normal and ER + tumor tissues using HCAb2. Methanol-acetone (1:1) fixed normal (a-d) and ER+ tumor tissues (e-h) were stained with HCAb2 and E-cadherin (epithelial marker). Matched samples represent normal and tumor tissues derived from the same patient. Arrows indicate cells with positive HCAb2 staining on cell surface. Scale bar represents 10 μm. i-l Immunofluorescence analysis of ER+ tumor tissues using HCAb2. ER+ tumor tissues were stained with HCAb2 and E-cadherin. HCAb2 showed positive staining of E-cadherin negative tumor cells. Panel j is magnified view of the inset shown in panel i. Scale bar represents 10 μm. m-r Immunofluorescence analysis of matched normal and triple negative tumor tissues using HCAb2. m-o Normal tissues were stained with HCAb2 and E-cadherin, while tumor tissues (p-r) were stained with HCAb2 and N-cadherin. For all samples nuclei were stained with DAPI and the scale bar represents 10 μm