Fig. 2

Partial Dll4/Notch inhibition due to haploid Dll4 deletion enhances less mature but productive angiogenesis in skin papillomas. a Vascular response examined by PECAM immunostaining indicating increased sprouting and network disorganization with reduced vessel calibers in Dll4^+/− vs. WT mice. b Mural cell coverage examined by double immunostaining of PECAM and α-SMA showing reduced recruitment of perivascular cells in Dll4^+/− tumors. c Tumor vessel competence evaluated by lectin perfusion and subsequent double staining to PECAM and biotinylated lectin demonstrating a reduction in the fraction of perfused vessels in Dll4^+/− vs. WT mice