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Fig. 2 | BMC Cancer

Fig. 2

From: Berberine, a natural compound, suppresses Hedgehog signaling pathway activity and cancer growth

Fig. 2

BBR displays selectivity for inhibiting Hh pathway activity. a The 293 T cells transfected with luciferase reporter activity containing NF-κB binding sites and Renilla-TK plasmids were exposed to TNF-α with BAY 11-872 or various concentrations of BBR for 6 h, and the cells were harvested for dual luciferase reporter activity analysis. The data are expressed as mean ± s.d., (n = 3) from three independent experiments. b The LS174T cells transfected with luciferase reporter constructs containing TCF/LEF binding sites and the Renilla-TK plasmids were exposed to PGE2 with various concentrations of BBR or H89 for 24 h, and the cells were collected for dual luciferase reporter activity analysis. The results are expressed as means ± s.d. from three independent experiments (n = 3). c The LS174T cells transfected with luciferase reporter constructs containing Gli binding sites and the Renilla-TK plasmids were exposed to PGE2 with various concentrations of BBR or GANT61 for 24 h, and the cells were collected for dual luciferase reporter activity analysis. Data are expressed as means ± s.d. from three independent experiments (n = 3). *p < 0.05; #p > 0.05

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