Fig. 4

Bcl2 interacts with mtAPE1 via its BH domains in mitochondrial extracts. Co-immunoprecipitation (co-IP) experiments were performed in mitochondrial extracts isolated from H1299 cells expressing WT or each of the Bcl2 deletion mutants using APE1 antibody. Normal rabbit IgG was used for negative control for co-IP. APE1-associated Bcl2 and total mtAPE1 in mitochondrial extracts were analyzed by Western blot