Fig. 3

Bcl2 is co-localized with APE1 via BH domains in mitochondria. a. Subcellular fractionation was performed in H460 and BEAS-2B cells to isolate heavy membrane (HM), light membrane (LM), cytosol (Cyt) and nuclear (Nuc) fractions. Bcl2 and APE1 in each fraction were analyzed by Western blot. Prohibitin and PCNA were used as mitochondrial and nuclear markers, respectively. b. APE1 and Bcl2 were analyzed in H460 cells expressing high levels of endogenous Bcl2 and APE1 using QD-IHF and quantified as described in “Methods”. Co-localization of APE1 and Bcl2 in mitochondria was analyzed and quantified with Nuance software in 10 randomly selected fields