Table 2 Primary antibodies used in this study
Antigen | Marker | Antibody | Dilution | Vendor |
|---|---|---|---|---|
CD68 | Pan-MΦ | Mouse anti-hCD68 Mab | 1:80 | Dako, Glostrup, Denmark |
CD80 | M1 MΦ | Mouse anti-hCD80 Mab | 1:200 | R&D systems, Minneapolis, MN, USA |
CD163 | M2 MΦ | Mouse anti-hCD163 Mab | 1:200 | Leica, Wetzlar, Germany |
CD4 | Th | Mouse anti-hCD4 Mab | 1:100 | NordiQC organization, Aalborg Denmark |
CD8 | CTL | Mouse anti-hCD8 Mab | 1:100 | Dako, Glostrup, Denmark |
CCR4 | Th2 | Mouse anti-hCCR4 Mab | 1:100 | Novus biological, Littleton, CO, USA |
CCR5 | Th1 | Rabbit anti-hCCR5 pab | 1:200 | Abcam, Cambridge, UK |
CXCR3 | Th1 | Mouse anti-hCXCR3 Mab | 1:200 | R&D systems, Minneapolis, MN, USA |
CXCL9 | Chemokine Th1 | Mouse anti-hCXCL9 Mab | 1:200 | R&D systems, Minneapolis, MN, USA |
STAT1 | IFNγ-inducible gene | Rabbit anti-hSTAT1 Pab | 1:200 | Santa Cruz, Santa Cruz, CA, USA |
Phospho-STAT1 | pSTAT1 (Tyr701) | Rabbit anti-pSTAT1 Mab | 1:50 | Cell signaling, Boston, MA, USA |