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Table 2 Primary antibodies used in this study

From: Tumor-associated macrophages in oral premalignant lesions coexpress CD163 and STAT1 in a Th1-dominated microenvironment

Antigen Marker Antibody Dilution Vendor
CD68 Pan-MΦ Mouse anti-hCD68 Mab 1:80 Dako, Glostrup, Denmark
CD80 M1 MΦ Mouse anti-hCD80 Mab 1:200 R&D systems, Minneapolis, MN, USA
CD163 M2 MΦ Mouse anti-hCD163 Mab 1:200 Leica, Wetzlar, Germany
CD4 Th Mouse anti-hCD4 Mab 1:100 NordiQC organization, Aalborg Denmark
CD8 CTL Mouse anti-hCD8 Mab 1:100 Dako, Glostrup, Denmark
CCR4 Th2 Mouse anti-hCCR4 Mab 1:100 Novus biological, Littleton, CO, USA
CCR5 Th1 Rabbit anti-hCCR5 pab 1:200 Abcam, Cambridge, UK
CXCR3 Th1 Mouse anti-hCXCR3 Mab 1:200 R&D systems, Minneapolis, MN, USA
CXCL9 Chemokine Th1 Mouse anti-hCXCL9 Mab 1:200 R&D systems, Minneapolis, MN, USA
STAT1 IFNγ-inducible gene Rabbit anti-hSTAT1 Pab 1:200 Santa Cruz, Santa Cruz, CA, USA
Phospho-STAT1 pSTAT1 (Tyr701) Rabbit anti-pSTAT1 Mab 1:50 Cell signaling, Boston, MA, USA
  1. CD cluster of differentiation, Mab monoclonal antibody, Pab polyclonal antibody, Th helper T lymphocyte, CTL cytotoxic T lymphocyte, CCR4 CC chemokine receptor 4, CXCR3 CXC chemokine receptor 3, CXCL9 CXC chemokine ligand 9, STAT1 signal transducer and activator of transcription