Fig. 1

Phenotype of Etv6-RUNX1, T2Onc, Pax5 leukemias. a Kaplan-Meier curves showing the tumor latency of ‘jumping’ Etv6^+/RUNX1, T2Onc^+/Tg, Pax5^+/− (ER, Onc, Pax) and Etv6^+/RUNX1, T2Onc^+/Tg (ER, Onc) mice, and ‘non-jumping’ control mice (Onc, Pax and Onc). b The presence of lymphoblasts in the peripheral blood (PB), spleen, bone marrow and liver from a representative mouse with leukemia. Magnification: peripheral blood smear (×400), upper row organs (×400) and lower row organs (×1,000). c Classification of the leukemias developed by mice shown in the Kaplan-Meier curve according to the Bethesda criteria for lymphoid and non-lymphoid murine malignancies. d Upper row: FACS plots from the bone marrow of a representative mouse demonstrate only background Gr-1/Mac-1 myeloid cells, with the majority of cells having a B220+/CD19+/surface Ig-. Middle and lower row: FACS plots from the bone marrow of a representative mouse demonstrate the B220+ cells to have a CD43+, CD127+, AA4.1+, CD24+, BP-1- phenotype. e Survival curves for SCID mice transplanted with 3.5-5 × 10⁵ B220+, CD19+ leukemia cells from ER, Onc, Pax mice and TAPJ23.1a which was an ER, Pax5 mouse. Each color represents a different ‘primary’ leukemia, as indicated by the “TAPJ” name of the mouse (n = 7)