Fig. 2
a Bak-dependent release of calcein from proteoliposomes is not activated by obatoclax or Noxa BH3 peptide. Assays ± tBid were conducted as described in Fig. 1d, in the presence of 1 μM obatoclax, Noxa BH3, or vehicle (0.05 % DMSO) alone. b As in Fig. 1d except proteoliposomes contained Bak and Mcl-1 derivatives and the assays conducted in the presence of 1 μM obatoclax or 1 μM des-methoxyl (DM) obatoclax. c As in Fig. 1d except that liposome-anchored Bcl-XL replaced Mcl-1 and ABT-737 was tested. Concentrations of assay constituents are provided in right panel. Shown are representative assays from at least 3 independent experiments