Fig. 2
From: Expression and role of oncogenic miRNA-224 in esophageal squamous cell carcinoma
Ectopic miR-224 expression changed the phenotypes of ESCC cell in vitro. a Cell viability CCK-8 assay. The effects of miR-224 on ESCC cell proliferation ability. b Colony formation assay. The effects of miR-224 on Eca109 cell line colony formation ability. c Flow cytometric apoptosis assay. miR-224I increased apoptosis of ESCC cell lines compared to the negative control. Apoptotic cells (%) = Q2 + Q3. Error bars represent the mean ± SD of three independent experiments. OD, optical density; miR-224 M, miR-224 mimic; miR-224I, miR-224 inhibitor; NC, negative control; *p < 0.05 and **p < 0.01