Fig. 1

Overexpression of miR-224 in esophageal intraepithelial neoplasia and ESCC tissues. a qRT-PCR. miR-224 expression was assessed in 40 pairs of IEN (intraepithelial neoplasia) biopsies and their matched adjacent normal tissues and 63 pairs of ESCC tissues and their matched normal esophageal tissues. Each point represents the mean of three independent experiments. The horizontal lines represent the median in each group. b Comparison of miR-224 expression in tissues obtained from ESCC and IEN patients. The horizontal lines represent the mean in each group. c Comparison of mean miR-224 expression stratified by TNM and pathologic classification. The horizontal lines represent the mean in each group. d qRT-PCR. Expression of miR-224 in esophageal cancer Eca109 and TE13 cell lines after transfected with miR-224 mimics or inhibitors. *p < 0.05 and **p < 0.01