Fig. 8

Xenografted CD140-CD49+ pMEC transduced with PGK-Tag-CMV-tdTomato injected subcutaneously with Matrigel or hydrogel produce epithelial neoplasms. a Section (H&E) from a CD140-CD49+ PGK-Tag-CMV-tdTomato tumor in Matrigel showing neoplastic glandular epithelium. Scale bar = 600 μm. b Magnified encircled area in (a). Scale bar = 100 μm. c Section (H&E) from a CD140-CD49+ PGK-Tag-CMV-tdTomato tumor in hydrogel showing neoplastic glandular epithelium. Scale bar = 600 μm. d Magnified encircled area in (c). Scale bar = 100 μm. Immunolocalization of (e) cytokeratins 8/18, (f) Ki67 and (g) estrogen receptor in CD140-CD49+ PGK-Tag-CMV-tdTomato tumorous pMEC injected with Matrigel. Scale bar = 100 μm. h CD140- pMEC transduced with PGK-Tag-CMV-tdTomato develop ductal-like structures as subcutaneous xenografts. Section (H&E) depicting scattered glandular epithelium with hollow or cell-filled lumens. Scale bar = 600 μm. i Magnified encircled area in (h). Scale bar = 100 μm. j CD140-tdTomato + pMEC transduced with PGK-Tag-CMV-tdTomato produce epithelial-type neoplasms in mammary fat pads. Section (H&E) depicting fibrosis with neoplastic squamous epithelium. Scale bar = 600 μm. k Magnified encircled area in (j). Scale bar = 100 μm