Fig. 7
From: Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo
PGK-Tag transduced pMEC have altered oncogene expression. a Proteins from pMEC (n = 3), pMEC-PGKTag (n = 4) and an NSG mouse tumor from injection of pMEC-PGK-Tag-CMV-tdTomato cells (n = 1) were analyzed by western blot for ST, MT and LT. b Western blot of the same proteins using b) anti-MAPK1/3, anti-phospho MAPK1/3, anti-cyclin D1 and anti-β-actin antibodies, and c) anti-Rb, anti-phospho Rb, anti-p53 and anti-β-actin. d Protein levels from blots in b) and c) were quantified for pMEC (n = 3) and pMEC-PGKTag cells (n = 5)