Fig. 5

PGK-Tag transformed pMEC promote the precocious development of dense epithelial structures that resemble terminal ductal lobular units (TDLU). a Whole mounts of mammary glands injected with either pMEC transduced with PGK-Tag lentivirus or non-transduced pMEC (control mammary gland). b Densely packed structures identified by whole mount analysis of PGK-Tag engrafted mammary glands were sectioned and stained (H&E). A parenchyma-rich area from a contralateral control mammary gland is included for comparison. Scale bar =100 μm. c Magnification of H&E from (a) scale bar = 100 μm. d PCR detection of PGK-Tag in genomic DNA from paraffin sections of the mammary gland shown in (b). Positive control is genomic DNA from pMEC transduced with PGK-Tag. Negative is ddH₂O template