Fig. 3From: Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivoInjection of lentivirus into the pig mammary gland. a Glands were injected intraductally (n = 9 pigs) with CMV-tdTomato, EF1α-tdTomato or PGK-tdTomato lentivirus with or without polybrene and harvested 5, 10 or 15d later. Lentiviral integration was determined by qPCR for tdTomato, corrected for 18S ribosomal RNA levels and expressed as a ratio of tdTomato integration with or without polybrene. Data are means ± SEM (n = 6-7). b Glands were injected intraductally (n = 9 pigs) with CMV-tdTomato, EF1α-tdTomato or PGK-tdTomato lentivirus and polybrene and harvested 5, 10 or 15d later. c Injections were into the mammary parenchyma (n = 9 pigs) with CMV-tdTomato, EF1α-tdTomato or PGK-tdTomato lentivirus and polybrene and harvested 5, 10 or 15d later. Negative controls (Neg) are genomic DNA from the mammary glands of two untreated pigs. Positive controls (Pos) are two pMEC lines transduced with CMV-tdTomatoBack to article page