Fig. 3

Extracellular vesicle mediated changes in cellular protein expression. EVs were isolated from malignant HCT116 cells. EVs were resuspended in PBS and co-cultured with non-malignant 1459 cells and malignant HCT116 cells. Upon completion of the 7-day co-culture, whole cell lysates were prepared for Western blot analysis, as reported. Western blot analysis results show and increased expression of Raf-1, pRKIP, STAT1, Prohibitin and 14-3-3 zeta/delta in 1459 + HCT116 EV co-cultures, as compared to 1459 control. A slight decrease expression of RKIP is also shown in this comparison. We examined these proteins based on LC-MS data (Table 1)