Fig. 4

CDH1 and PPARA as direct targets of miR-9 in HCC. a Sequence complementarity between miR-9 seed sequence and the 3′UTRs of PPARA and CDH1. b CDH1 and PPARA 3′UTR luciferase assay activity in SNU-449 cells transfected with miR-Ctrl or miR-9, 48 h post-transfection. MiR-9 sequence was wildtype or mutated (miR-9 mut). c CDH1 mRNA levels in SNU-449 and HepG2 cells transfected with miR-9 or anti-miR-9, 48 h post-transfection, assessed by real-time RT-PCR. d Vimentin, (e) PPARA and (f) PDK4 mRNA levels in SNU-449 cells transfected with miR-9, 48 h post-transfection, assessed by real-time PCR. g CDH1 mRNA levels in SNU-449 and HepG2 cells transfected with an siRNA against PPARA (siPPARA) or an siRNA negative control (siCtrl), 48 h post-transfection. All data are represented as mean ± SE. ***P < 0.001, **P < 0.01, *P < 0.05