Table 3 MSP primer sequences used to detect GTSE1 DNA methylation
Methylation/ Unmethylation primers | Sequence |
|---|---|
Methylated GTSE1 forward | 5′-GTA GTG CGT ATG CGT ATT GGA C−3′ |
Methylated GTSE1 reverse | 5′-GCG AAT TAC CGA TTA ATC GAT−3′ |
Unmethylated GTSE1 forward | 5′-AGT AGT GTG TAT GTG TAT TGG ATG−3′ |
Unmethylated GTSE1 reverse | 5′-AAA ACA CAA ATT ACC AAT TAA TCA AT−3′ |