Fig. 3

M1 macrophage marker expression. THP-1 macrophages were incubated with IFN-γ (20, 50, 100 ng) and/or LPS 10 pg/ml during 24 h. (a) mRNA expression of M1 macrophage markers was studied by RT-qPCR and normalized by RPS9 expression. Results are expressed as means ± 1 S.D. (n = 3). (b) IL-6 and CXCL10 secretion in culture medium was measured by ELISA. Results are expressed as means ± 1 S.D. (n = 3). Statistical analysis was carried out with a one-way ANOVA test followed by a Holm-Sidak post-test. NS: not significantly different. *, ** or ***: significantly different from the corresponding control (Ctrl) respectively with p < 0.05, 0.01 or 0.001; ###: significantly different with p < 0.001. Statistical analyses were performed on non-transformed data for TNF-α (a), IL-1ß (a), IL-6 (b) and CXCL10 (b) and on log-transformed data for IL-6 (a), CXCL10 (a), CD80 (a) and HLD-DR (a)