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Fig. 5 | BMC Cancer

Fig. 5

From: P-glycoprotein confers acquired resistance to 17-DMAG in lung cancers with an ALK rearrangement

Fig. 5

Induction of P-gp/MDR1 expression in paclitaxel-resistant cells. a The response to paclitaxel was assessed as described in Fig. 1. b P-gp expression was analyzed using western blot analysis. c Cells were pretreated with or without 5 μM verapamil and then treated with the indicated concentrations of paclitaxel. After 72 h, cell viability was measured using the MTT assay. d Control or P-gp siRNAs (100 nM) were introduced into resistant cells, and P-gp silencing was confirmed by western blot analysis. e, f and g Cells were treated with the indicated concentrations of 17-DMAG or AUY922 after transfection of P-gp siRNA or pretreatment with 5 μM verapamil. Cell viability was measured 72 h later using the MTT assay

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