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Fig. 3 | BMC Cancer

Fig. 3

From: Nrf2 is a potential prognostic marker and promotes proliferation and invasion in human hepatocellular carcinoma

Fig. 3

Modulation of endogenous Nrf2 expression. a After transfected with Nrf2-shRNA (shRNA-867, shRNA-1118, shRNA-1757, or shRNA-2019) or control shRNA (shNC), expression levels of Nrf2 mRNA in Bel-7402 cells were detected by qRT-PCR; b-c After transfected with Nrf2-shRNA (shRNA-867, shRNA-1118, shRNA-1757, or shRNA-2019) or control shRNA (shNC), expression levels of Nrf2 protein in Bel-7402 cells were detected by western blot; d After transfected with Nrf2 expression plasmid (pEFGP-Nrf2-1 or pEFGP-Nrf2-2) or mock pEGFP plasmid (pEGFP-NC), expression levels of Nrf2 mRNA in HepG2 cells were detected by qRT-PCR; e-f After transfected with Nrf2 expression plasmid (pEFGP-Nrf2-1 or pEFGP-Nrf2-2) or mock pEGFP plasmid (pEGFP-NC), expression levels of Nrf2 protein in HepG2 cells were detected by western blot. *P <0.05 compared with control (Bel-7402 cells or HepG2 cells respectively) or shNC and pEGFP-NC

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