Fig. 6

Inhibition of the p70S6K/glycolysis pathway plays an important role in Dex re-sensitization by Rap. a After 48 h exposure to Rap and/or Dex, cells were lysed and extracts were analyzed by western blotting for p-p70S6K and p-AMPK. Quantification is shown as a ratio of phospho-protein to total protein. b After 48 h exposure to Rap and/or Dex, glucose consumption and lactic acid production were measured with the Glucose (HK) Assay Kit and Lactic Acid Assay Kit, respectively. c After 48 h exposure to 2-DG and/or Dex, glucose consumption and lactic acid production were measured with the Glucose (HK) Assay Kit and Lactic Acid Assay Kit, respectively. d The viability rates of the cells were evaluated by MTT assays after 48 h exposure to 2-DG and/or Dex. e The early stage of apoptosis was detected by Annexin V-FLUOS/PI staining (Annexin V-FLUOS positive/PI negative) after 48 h exposure to 2-DG and/or Dex. f The cell cycle phases were analyzed by PI staining after 48 h exposure to 2-DG and/or Dex. *: p < 0.01 versus the control group, Dex group, or Rap group. R, Rap; D, Dex; RD, Rap + Dex and C, control