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Fig. 5 | BMC Cancer

Fig. 5

From: Targeting mTOR/p70S6K/glycolysis signaling pathway restores glucocorticoid sensitivity to 4E-BP1 null Burkitt Lymphoma

Fig. 5

The combination of Rap with Dex induces autophagic cell death. a After 24 h exposure to Rap and/or Dex, ultrastructural changes were examined by transmission electron microscopy. Arrows indicate the autophagic vacuoles in the R and RD groups (8000× magnification). b Cells were lysed and extracts were analyzed by western blotting for LC3. The experiments were repeated three times and the data show the representative results. c MDC staining revealed the formation of autophagosomes (1000× magnification). d The cells were pretreated with 1 mM 3-MA or 0.1 % DMSO as a control for 2 h in four groups. The viability rates of the cells were evaluated by MTT assays. *: p < 0.05 versus control in the R and D groups. e Apoptosis was detected by Annexin V-FLUOS/PI staining (Annexin V-FLUOS positive/PI negative). R, Rap; D, Dex; RD, Rap + Dex and C, control

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