Digital imaging in the immunohistochemical evaluation of the proliferation markers Ki67, MCM2 and Geminin, in early breast cancer, and their putative prognostic value

Table 3 Immunohistochemical expression of Ki67, MCM2 and Geminin in 309 cases of early breast cancer as assessed by light microscope and digital imaging and the correlation between the two methods of scoring

Marker	Score	Conventional method of scoring				Digital method of scoring				Correlation
		Available values	Max	Min	Median	Available values	Max	Min	Median	Spearman’s co-efficient
Ki67	Maximum of the 3 cores	258	95	0	10	175	97	0	7	n = 174^a
										0.90 (0.86−0.92)
										p < 0.001
	Average of the 3 cores	258	90	0	7.7	175	85.33	0	4.5	n = 174^a
										0.91 (0.88−0.93)
										p < 0.001
MCM2	Maximum of the 3 cores	260	100	0	30	167	100	0	37	n = 167^a
										0.92 (0.90−0.94)
										p < 0.001
	Average of the 3 cores	260	100	0	24	167	98.5	0	27	n = 167^a
										0.94 (0.91−0.95)
										p < 0.001
Geminin	Maximum of the 3 cores	258	40	0	5	270	62	0	2	n = 257
										0.88 (0.85−0.91)
										p < 0.001
	Average of the 3 cores	258	28.3	0	3	270	37	0	2	n = 257
										0.90 (0.87−0.92)
										p < 0.001

^aThe number of cores available for digital scoring was not the same as the number available for scoring conventionally. Hence, only those scored by both techniques were compared with each other

ISSN: 1471-2407