Table 4 Detailed bioinformatic characteristics of TMEMs with topology and function predictions. ¹ - Metaserver prediction. ² - Localizome prediction, ³ - PSORT II prediction. ⁴ - Functional data available; protein localized in Golgi apparatus, endosomes and lysosomes. GI - GenInfo Identifier. TM - transmembrane helix segment. ER - endoplasmic reticulum. Mit – mitochondrial. Nuc – nuclear. Cyt – cytoplasmic. n/a - no result

Full name

transmembrane protein 213 precursor

transmembrane protein 207 precursor

transmembrane protein 61

transmembrane protein 45B

transmembrane protein 30B

receptor-transporting protein 3

transmembrane protein 72

transmembrane protein 116 isoform 1

solute carrier family 35 member G2

transmembrane protein 45A

Alternative names

–

UNQ846

–

–

CDC50B

LTM1; TMEM7

KSP37; C10orf127; bA285G1.3

–

TMEM22

DERP7

NP (NCBI)

001078898.1

997199.1

872338.1

620143.1

001017970.1

113628.1

001116848.1

001180460.1

079522.2

060474.1

GI

146229352

46409276

32698902

20270331

63003930

13899263

183227675

302058299

148224156

8922243

Protein isoforms

1

1

1

1

1

1

1

3

1

1

Transcript variants

1

1

1

1

1

1

1

3

3

1

Human length (aa)

107

146

210

275

351

232

275

337

412

275

Pfam

TMEM213 family in eukaryotes; 154 aa; unknown function

n/a

TMEM61 family in eukaryotes; 150–211 aa; unknown function

DUF716 family in metazoa (unknown function) and plants (response to viral attack)

CDC50/ LEM3 (ligand-effect modulator 3) family in glucocorticoid receptor pathway

zf-3CxxC family with zinc-binding domain

n/a

n/a

EamA drug/metabolite transporter-like family with two copies of EamA domain

DUF716 family in metazoa (unknown function) and plants (response to viral attack)

Tertiary structure prediction¹

n/a

Similar to TM protein stannin (PDB Id: 1zza)

n/a

n/a

n/a

Similar to Zn-binding proteins (PDB Id: 2cup; 2dkt; 2e2z; 3hcs)

n/a

Similar to human adrenoreceptor (PDB Id: 2rh1; 2r4r) and human adenoreceptor (PDB Id: 3eml)

Similar to TM domain of the multidrug-resistance antiporter from E. coli EmrE (PDB Id: 3b5d)

n/a

TM domains

Predicted topology³

Type 1a with cyt tail (92 to 107)

Type 1a with cyt tail (71 to 146)

Type 3a

Type 3b

Type 3a

Type Nt with cyt tail (1 to 211)

Type 2 with cyt tail (1 to 94)

Type 3a

Type 3a

Type 3a

Cleavable N-terminal signal peptide³

Yes (1 to 27)

Yes (1 to 29)

No

No

No

No

No

Yes (1 to 50)

No

No

Cytoplasmic/ Nuclear discrimination³

Nuc (55.5)

Cyt (94.1)

Nuc (70.6)

Cyt (94.1)

Cyt (89)

Cyt (89)

Cyt (94.1)

Cyt (94.1)

Cyt (94.1)

Cyt (94.1)

Subcellular localization³

ER (44.4 %)

ER (44.4 %)

Mit (39.1 %) Nuc (34.8 %)

ER (66.7 %)

ER (39.1 %) Mit (39.1 %)

Cyt (30.4 %)

Mit (30.4 %)

ER (43.5 %)

ER⁴ (55.6 %)

ER (77.8 %)