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Fig. 9 | BMC Cancer

Fig. 9

From: A potential small-molecule synthetic antilymphangiogenic agent norcantharidin inhibits tumor growth and lymphangiogenesis of human colonic adenocarcinomas through blocking VEGF-A,-C,-D/VEGFR-2,-3 “multi-points priming” mechanisms in vitro and in vivo

Fig. 9

The expression of VEGF-A, VEGF-C, VEGF-D, VEGFR-2 and VEGFR-3 proteins/mRNAs of HCACCs and the co-culture system and the effect of NCTD on expression of these proteins/ mRNAs by western blotting (a and b) and fluorescent quantitative RT-PCR (c and d) in vitro. a and b Protein expression by western blotting: the expression of VEGF-C, VEGF-D and VEGFR-3 proteins of the co-culture system was higher than those of alone HCACC culture (*P = 0.001), but there was no difference on VEGF-A and VEGFR-2 expression between alone HCACC culture and the co-culture system. After treatment with NCTD, mF4-31C1 or NCTD + mF4-31C1, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins of the co-culture system was downregulated significantly as compared to control group (all *P < 0.01), with lowest expression of these proteins in NCTD + mF4-31C1 group (#P < 0.01, vs. NCTD or mF4-31C1 group); whereas the expression of VEGF-A and VEGFR-2 proteins in NCTD or NCTD + mF4-31C1 group was lower than those of control or mF4-31C1 group (*P < 0.01), but there was no difference on VEGF-A and VEGFR-2 expression between control group and mF4-31C1 group, or between NCTD group and NCTD + mF4-31C1 group. c and d mRNA expression by RT-PCR: the expression of VEGF-C, VEGF-D and VEGFR-3 mRNAs of the co-culture system was higher than those of alone HCACC culture (*P = 0.001); but there was no difference on the expression VEGF-A and VEGFR-2 mRNAs between alone HCACC culture and the co-culture system. After treatment with NCTD, mF4-31C1 or NCTD + mF4-31C1, the expression of VEGF-C, VEGF-D and VEGFR-3 mRNAs of the co-culture system was downregulated significantly as compared to control group (all *P < 0.01), with lowest expression of these mRNAs in NCTD + mF4-31C1 group (#P < 0.01, vs. NCTD or mF4-31C1 group); whereas the expression of VEGF-A and VEGFR-2 mRNAs in NCTD or NCTD + mF4-31C1 group was lower than those of control or mF4-31C1 group (*P < 0.01), but there was no difference on the expression of VEGF-A and VEGFR-2 mRNAs between control group and mF4-31C1 group, or between NCTD group and NCTD + mF4-31C1 group

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BMC Cancer

ISSN: 1471-2407

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