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Fig. 4 | BMC Cancer

Fig. 4

From: A potential small-molecule synthetic antilymphangiogenic agent norcantharidin inhibits tumor growth and lymphangiogenesis of human colonic adenocarcinomas through blocking VEGF-A,-C,-D/VEGFR-2,-3 “multi-points priming” mechanisms in vitro and in vivo

Fig. 4

NCTD inhibit lymphatic tube formation of the 3-D culture of HDLECs or the 3-D co-culture system in vitro. a Capillary-tube formation and capillary-tube number of each group under an inverted light microscope (magnification × 200). When seeded on the lower compartment of the chamber coated with Matrigel matrix for one week, HDLECs formed typical capillary-like tubes with pipe wall, the lumen and progressive branches, while the capillary tube formation was more obvious in the upper compartment of the chamber with HT-29 cells than without (§P < 0.01). After treatment with NCTD, mF4-31C1 or NCTD+ mF4-31C1, HDLECs didn’t form above capillary-like tube, with visible cell aggregation, float, nuclear fragmentation, apoptosis; and the capillary-tube number in these groups was markedly decreased as compared to control group (*P < 0.000), while this number in NCTD or NCTD+ mF4-31C1 group was less than that of mF4-31C1 group (all #P < 0.01). b The expression of LYVE-1 and D2-40 from the 3-D co-culture system in vitro using western blotting. The positive expression of LYVE-1 and D2-40 proteins was observed in control group; but LYVE-1, D2-40 protein expression was significantly downregulated in mF4-31C1, NCTD or NCTD + mF4-31C1 group (#P < 0.01)

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BMC Cancer

ISSN: 1471-2407

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