Fig. 6

CRIF1 is required for normal expression of mitochondrion-encoded proteins. Comparisons are made between Jcam cells expressing scrambled shRNA (Control) and CRIF1-specific shRNA (CRIF1 KD). (a) Normalized whole cell lysates were subjected to immunoblotting for CRIF1, ND1, COI, COIV, VDAC1 and GAPDH. Signal intensity was quantitated for CRIF1, ND1 and COI and fold change is indicated below the images. (b) Total RNAs were subjected to real-time PCR using primers specific for human CRIF1, ND1, COI and COIV. Data from triplicates were normalized to actin and expressed as fold change of CRIF1 knock-down (CRIF1 KD) in comparison to control Jcam. (c, d, e) Mitochondrial membrane potentials, oxygen consumption rate, and mitochondrial superoxide levels were analyzed as described for Fig. 2. Data are presented as percentage of change from CRIF1 knock-down in comparison to control Jcam. Statistical analyses show the results from three independent studies, *p < 0.05, ***p < 0.001