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Fig. 4 | BMC Cancer

Fig. 4

From: The combination of intravenous Reolysin and gemcitabine induces reovirus replication and endoplasmic reticular stress in a patient with KRAS-activated pancreatic cancer

Fig. 4

IHC analysis of GRP78/BiP and NOXA expression in biopsy specimens taken prior to (baseline) and following treatment with Reolysin and gemcitabine. IHC was performed on paraffin-embedded tumor sections followed by heat-induced epitope retrieval. (Left) GRP78/BiP, a marker of ER stress induction, is induced following treatment. NOXA, a pro-apoptotic gene, is also significantly increased after treatment. (Right) Quantification of the relative intensity of IHC staining in the biopsy specimens was performed using ImageJ software. Mean ± SD, n = 3. *Indicates a significant difference compared to baseline, p < 0.05

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