Fig. 3

Co-expression of reoviral protein and caspase-3 is consistent with productive lytic infection in the patient’s pancreatic cancer cells when treated with intravenous Reolysin and gemcitabine. Blue indicates the nucleus of the cancer cell. Fluorescent green is the reoviral protein and fluorescent red is active caspase-3 protein. Yellow represents co-localization of reovirus and active caspase-3 in the same cancer cells after co-expression IHC analysis. The Nuance system converts each signal to a fluorescent-based signal to determine co-expression of the two targets of interest