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Fig. 4 | BMC Cancer

Fig. 4

From: Expression of an estrogen-regulated variant transcript of the peroxisomal branched chain fatty acid oxidase ACOX2 in breast carcinomas

Fig. 4

ACOX2-i9 knockdown reduces growth of T47D and MDA-MB 436 cells. Colony formation assay of T47D cells stably expressing shRNA targeting full length ACOX2 (shRNA N and N’), full length and ACOX2-i9 transcripts (shRNA C and C’), or empty vector (control) (a and b). Colony formation assay of MDA-MB 436 cells (g) using the N’, C’, and control constructs. Cells were methanol fixed and stained with Chrystal Violet. Colonies were counted manually (c, d, and h). Bars are average of three experiments performed in triplicates (+/− SE), *p < 0.05 as assed by two-sided t-test. Knockdown was assessed by Western blotting (e, f, and i)

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