Fig. 3

Estrogen regulation of ACOX2-i9 in cell lines. Western blot analysis was performed on whole cell lysates from T47D cells either depleted of estradiol for 72 h, treated with 4-OHT for 48 h, or treated with fulvestrant for 48 h (a), or treated with increasing doses of PPT for 48 h (b). MDA-MB 436 cells were either depleted of estradiol for 72 h, treated with 4-OHT for 48 h, or treated with fulvestrant for 48 h (c), or treated with PPT for 48 h (d), HepG2 cells were treated with 4-OHT for 48 h (e) (see Methods for details). Blots were probed with the C-terminal ACOX2 and Tubulin antibodies