Fig. 2

Expression of ACOX2 in breast cancer cell lines. ACOX2 and ACOX2-i9 mRNA levels were assessed by qRT-PCR in HepG2 cells and breast cancer cell lines (a), quantification is shown relative to HepG2 expression. Protein extracts from HepG2 and breast cancer cell lines were probed with a C-terminal antibody against ACOX2 (b). Highly sensitive chemiluminescent substrate (c) was included for illustration purposes to show even low levels of protein expression. HepG2 cells were transfected with shRNA targeting the N-terminal and C-terminal regions of ACOX2 (d)