Fig. 4

Paclitaxel down-regulates miR-9 expression in EOC cells through influencing DNA methylation. a. BSP results of the hsa-miR-9-1 CpG island region in different ovarian carcinoma cell lines before and after 24 h paclitaxel treatment. 10 clones were sequenced for each cell line. Each circle indicates a CpG dinucleotide, black circle: methylated CpG; open circle: unmethylated CpG. b. BSP results of the hsa-miR-9-2 CpG island region in different ovarian carcinoma cell lines before and after 24 h paclitaxel treatment. 10–12 clones were sequenced for each cell line. c. BSP results of the hsa-miR-9-3 CpG island region in different ovarian carcinoma cell lines before and after 24 h paclitaxel treatment. 10–11 clones were sequenced for each cell line. d. Real-time RT-PCR analysis of miR-9 in SKOV3 and A2780 cell lines treated with paclitaxel at different time. The experiments were repeated three times