Fig. 2
The TRAIL receptor preference for apoptosis induction is variable in pancreatic cancer cells. a-e Supernatants from HEK293 cells that were transfected with the EGFP control expression construct (ctrl; grey; 1:2 diluted), sTRAILwt (yellow; 2 ng/ml), sTRAILDR5–1 (dark green; 2 ng/ml), sTRAILDR5–2 (light green; 2 ng/ml), sTRAILDR4–1 (dark blue; 2 ng/ml), sTRAILDR4–2 (light blue; 2 ng/ml) or sTRAILDR4–3 (blue-grey; 2 ng/ml) were then transferred onto (a) HeLa cells (prototypic DR4 specific cell type), (b) Colo205 cells (prototypic DR5 specific cell type), (c) Colo357 pancreatic cancer cells, (d) BxPC-3 pancreatic cancer cells and (e) AsPC-1 pancreatic cancer cells. After 24 h apoptosis was measured