Fig. 1

PPARα domain structure and probe locations. PPARα-wild type (wt) and truncated (tr) transcripts are shown as lines with primers and probes for TaqMan gene expression assays indicated schematically above, and siRNA probes below. The generation of an alternative splice variant via introduction of a pre-mature stop codon (asterisk) is shown with the dotted line. The corresponding protein products of two splice isoforms are underlined. The indicated protein domains are: AF-1, activation function 1 domain, DBD, DNA-binding domain, LBD, ligand-binding domain. “AB” indicates the region of antibody binding