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Fig. 7 | BMC Cancer

Fig. 7

From: Estrogen receptor α enhances the transcriptional activity of ETS-1 and promotes the proliferation, migration and invasion of neuroblastoma cell in a ligand dependent manner

Fig. 7

Effect of estrogen and ERα on SH-SY5Y cells proliferation and anchor-independent growth. SH-SY5Y cells, which were cultured in phenol red-free DMEM added 2 % charcoal-stripped FBS (a and b) or in normal DMEM added 10 % normal FBS (c and d), were treated with E2 (100nM) or ICI-182780 (300nM). Cells were then measured by MTT assay (a-d) or soft agar assay (e). Colony was shown in the photographs (e). (a-d, f) Data are mean ± SD of triplicate independent experiments and have been repeated 3 times with similar numbers. The effect of Estrogen on ETS-1 targeted genes MMP1 or MMP9 was detected by Western blot (g). *P < 0.05 versus Solvent control (DMSO) or E2; *P < 0.05 versus Solvent control (DMSO) or ICI-182780; *P < 0.05 versus with E2 or ICI-182780

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BMC Cancer

ISSN: 1471-2407

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