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Fig. 6 | BMC Cancer

Fig. 6

From: Estrogen receptor α enhances the transcriptional activity of ETS-1 and promotes the proliferation, migration and invasion of neuroblastoma cell in a ligand dependent manner

Fig. 6

Estradiol modulated the recruitment of ETS-1 and transcriptional co-regulator to mmp1 promoter region. a The recruitment of ETS-1, ERα, SRC-1 and AIB-1 to the mmp1 promoter was detected by ChIP assay. b The recruitment of ETS-1, ERα, NCoR and SMRT to the mmp1 promoter was detected by ChIP assay. (c-d) SH-SY5Y cells were stimulated with 10nM E2 for 1 h. SH-SY5Y cells were transfected with SRC-1 (a), AIB-1 (a), NCoR-1 (b), or SMRT (b) expression vectors or empty vectors. Cells were then harvested for the luciferase assay. The values are the mean ± SD from three independent experiments. Western blot (bottom) indicates the expression level of proteins with anti-SRC1, anti-AIB1, anti-NCoR, or anti-SMRT antibodies. GAPDH was used as loading control. *P < 0.05

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BMC Cancer

ISSN: 1471-2407

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